Pantoea agglomerans foreign bodyinduced septic arthritis. The api20e biochemical kit system biomerieux and manual biochemical. Full text endophthalmitis caused by pantoea agglomerans. Isolation of endophytic diazotroph pantoea agglomerans and. Pantoea agglomerans is a gramnegative rod in the enterobacteriaceae family. Pantoea ananatis causes disease symptoms in a wide range of economically important agricultural crops and forest tree species worldwide. Phylogeny and identification of pantoea species and typing of. Group 1 included 21 hiv positive patients with acute respiratory syndrome. Pantoea is a genus of gramnegative bacteria of the family erwiniaceae, recently separated from the genus enterobacter. So, this study was conducted to isolate and identifypantoea spp from different sources clinical and environmental by different methods and detect the susceptibility of bacteria to different type of antibiotic.
The genus pantoea includes several species that are generally associated with plants, either as epiphytes or as pathogens, and some species can cause disease in humans. Isolation, identification and antimicrobial susceptibility of pantoea. Unrooted phylogenetic tree based on a comparison of the 16s ribosomal dna sequence of pantoea agglomerans lma2 gq478021 and some of their closest phylogenetic relatives validly published strains, the tree was created by the neighborjoining method, the numbers on the tree indicates the percentages of bootstrap sampling derived from 1,000 replications, bar inferred. Pantoea agglomerans formerly enterobacter agglomerans is a gramnegative aerobic bacillus in the family enterobacteriaceae. Pantoea agglomerans in immunodeficient patients with. Pronunciation of pantoea with 2 audio pronunciations, 1 translation and more for pantoea. Prevelance and antibiotic susceptibility patterns of pantoea spp. The enterobacterial genus pantoea contains both freeliving and hostassociating species, with considerable debate as to whether.
Attachment was rapid and reached equilibrium after contact for 60 min. We characterized 36 pantoea strains, including 28 strains of diverse origins initially identified as p. The 16s rdna gene sequence of isolate m009 showed the highest sequence similarity to pantoea stewartii s9116, which is a plant pathogen. Unusual causes of peritonitis in a peritoneal dialysis. Pantoea ananatis is found in a wide range of natural environments, including water, soil, as part of the epi and endophytic flora of various plant hosts, and in the insect gut. Pantoea ananatis erwinia ananas erwinia uredovora causes pink disease in pineapple ananas comosus, leafspot in syngonium syngonium podophyllum, purple stain in papaya carica papaya, and cereal rust. Isolation of a rice endophytic bacterium, pantoea sp.
Unrooted phylogenetic tree based on a comparison of the 16s ribosomal dna sequence of pantoea agglomerans lma2 gq478021 and some of their closest phylogenetic relatives validly published strains, the tree was created by the neighborjoining method, the numbers on the tree indicates the percentages of bootstrap sampling derived from 1,000 replications, bar inferred nucleotide. Pantoea agglomerans is a plant pathogen which very rarely causes an opportunistic infection. The patient underwent arthroscopy with removal of a thorn. Pathogenic isolates have been found to infect insects, plants and humans, yet it is unclear whether these isolates have similar pathogenic potential to the free living environmental populations. Pantoea dispersa it the next emerging monster in our intensive care units. Quantitative evaluation of the hostcolonizing capabilities. Pantoea agglomerans, the pantoea species most commonly isolated from humans, is widely distributed in nature and has been isolated from numerous ecological niches, including plants, water, soil, humans, and animals. Since 2016, new symptoms of browntoslightly reddish spots on the upper blades of the leaves, finally causing leaf blight on rice oryza sativa. All species of the genus pantoea can be isolated from feculent material, plants, and soil, where they can be either pathogens or commensals. Pantoea agglomerans an overview sciencedirect topics. Apr 10, 2011 an 87 yearold female who was undergoing peritoneal dialysis presented with peritonitis caused by alcaligenes faecalis and pantoea agglomerans in consecutive years. Modeling the rate of attachment of listeria monocytogenes. First report of pantoea agglomerans on oryza sativa in turkey.
The most prominent species of the genus is pantoea agglomerans, formally known as enterobacter agglomerans and earlier referred to as erwinia herbicola. Some strains have proven effective as biological control agents and plantgrowth promoters, while other strains have been implicated in diseases of a broad range of plant hosts and humans. Pantoea and xanthomonas species associated with blight and. The strain was also capable of reducing the lignin colour 524%. Pantoea agglomerans, a bacterium associated with plants,is not an obligate infectious agent in. Isolation, identification and antimicrobial susceptibility of. Pantoea dispersa it the next emerging monster in our. Pantoea species are primarily plant pathogens, but pantoea species have also been isolated endophytically and epiphytically from a wide variety of crops. Specific isolation and identification were done for pantoea spp. Dec 03, 2008 pantoea agglomerans and other pantoea species cause infections in humans and are also pathogenic to plants, but the diversity of pantoea strains and their possible association with hosts and disease remain poorly known, and identification of pantoea species.
Alistipes onderdonkii collinsella aerofaciens dorea formicigenerans ruminococcus torques eubacterium rectale phascolarctobacterium succi faecalibacterium prausnitzii subdoligranulum spp. To report the clinical findings, antibiotic sensitivities, and visual. Pantoea agglomerans usually occurs in plants as an epi or endophytic symbiont, often as mutualist. Pantoea bacteria are yellow pigmented, ferment lactose, are motile, and form mucoid colonies. Pantoea bacteria belong to a large group of organisms of the enterobacteriaceae family, also called enteric bacilli. This article highlights the need to search for a foreign body in pantoea septic arthritis. Liying wang 1,2, jin wang 3 and chuanyong jing 1,2 1 state key laboratory of environmental chemistry and ecotoxicology, research center for ecoenvironmental sciences, chinese academy of sciences, beijing, china. Prevelance and antibiotic susceptibility patterns of. The genus pantoea is a highly diverse group comprising free living, and both pathogenic and nonpathogenic hostassociating species.
Transfer of enterobacter agglomerans beijerinck 1888 ewing and fife 1972 to pantoea gen. Although we have found some differences in biochemical characteristics between 9c and pantoea spp. Department of energy joint genome institute, a doe office of science user facility, is supported by the office of science of the u. Molecular validation of clinical pantoea isolates identified by maldi.
Pantoea agglomerans, a gammaproteobacterium of plant origin, possesses many beneficial traits that could be used for the prevention andor treatment of human and animal diseases, combating plant pathogens, promotion of plant growth and bioremediation of the environment. Scanning electron microscopy of prepared leaf tissues has shown that bacteria can be found on the abaxial and. To the best of our knowledge this is the first case report of. Bacteria isolated from eucalyptus leaves and shoots showing symptoms of blight and dieback collected in uganda, uruguay and argentina and from maize displaying brown stalk rot symptoms in south africa were tentatively placed in the genus pantoea on the basis of phenotypic and biochemical tests. A new method to measure the probability of detachment was. Ultrasound examination revealed a foreign body in the synovial fluid. Pantoea agglomerans, a plant pathogen causing human disease. Two pantoea isolates of interest 4 and 5 were identified as pantoea ananatis based on both 16s sequence similarity to pantoea spp. Pantoea agglomerans and other pantoea species cause infections in humans and are also pathogenic to plants, but the diversity of pantoea strains and their possible association with hosts and disease remain poorly known, and identification of pantoea species is difficult. Extended genome report open access highquality draft genome sequence of a new phytaseproducing microorganism pantoea sp. Pantoea agglomerans infections in humans are uncommon. We report the case of a 25yearold, healthy male, who presented with indurated swelling over the posterolateral aspect of his right thigh, associated with pain for one month.
Pdf microbiological and molecular study has been carried on to perform isolation, identification and pcr detection of pantoea agglomerans. Most common infections reported are septic arthritis or synovitis. With the following report we discuss the importance of these unusual microorganisms in peritoneal dialysis patients. This organism is potential candidates as powdered infant milk formulaborne opportunistic pathogen. Pdf pantoea agglomerans, a plant pathogen causing human. Pantoea agglomerans annals of agricultural and environmental. Pantoea species causing early onset neonatal sepsis.
Pantoea agglomerans infection behaving like a tumor after. The common pathogens causing early onset neonatal sepsis are klebsiella pneumoniae, serratia marcescens, group b streptococcus, escherichia coli, coagulasenegative staphylococci and pseudomonas spp. The rate of attachment of bacteria to, and their subsequent detachment from, the cut surface of raw potato tissue was measured and modeled by using mathematical approaches that allowed detailed objective comparisons of adhesion processes under different conditions. Four hiv positive patients with tuberculosis symptoms were included in group 2.
An 87 yearold female who was undergoing peritoneal dialysis presented with peritonitis caused by alcaligenes faecalis and pantoea agglomerans in consecutive years. Pantoea agglomerans and other pantoea species cause infections in humans and are also pathogenic to plants, but the diversity of pantoea strains and their possible association with hosts and disease remain poorly known, and identification of pantoea species. Pantoea spp in addition, there are very little information regarding the role ofpantoea spp in human bacterial infections. May 27, 2014 pantoea ananatis is found in a wide range of natural environments, including water, soil, as part of the epi and endophytic flora of various plant hosts, and in the insect gut. Pantoea agglomerans and other pantoea species cause infections in humans and are also. Isolation and characterization of plant growth promoting. This species has been identified as a possible cause of equine abortion and placentitis and a haemorrhagic disease in dolphin fish coryphaena hippurus. Pantoea agglomerans, a gammaproteobacterium of plant origin, possesses many beneficial traits that could be used for the prevention. It is regarded as an emerging pathogen based on the increasing number of reports of diseases occurring on previously unrecorded hosts in different parts of the world. Shigella flexneri bifidobacterium pseudocate proteus mirabilis bacteroides vulgatus bacteroides ovatus pantoea spp. It is regarded as an emerging pathogen based on the. It is regarded as an emerging pathogen based on the increasing number of reports of diseases occurring on previously unrecorded hosts in.
Pantoea agglomerans is a gramnegative bacterium that belonging to the family erwiniaceae it was formerly called enterobacter agglomerans, or erwinia herbicola and is an ubiquitous bacterium commonly isolated from plant surfaces, seeds, fruit, and animal or human feces and can be found throughout a honeybees environment. Comparative genomic analysis reveals organization, function and evolution of ars genes in pantoea spp. Effects of postharvest onion curing parameters on bulb rot. Phylogeny and identification of pantoea species and typing.
A variety of species were collected from nonsurface sterilized p. Highquality draft genome sequence of a new phytase. Synovial fluid culture yielded pantoea agglomerans identified by 16s rdna polymerase chain reaction. Pantoea agglomerans, a bacterium associated with plants, is not an obligate infectious agent in humans. The genus pantoea forms a monophyletic unit closely related to erwinia. All members of enterobacteriaceae are gramnegative bacilli or coccobacilli ranging from 0. The aim of this paper was to determine in 32 patients from 4 different mexican hospitals the frequency of opportunistic bacteria in the 2010 to 2011 time period. Comparative genomic analysis reveals organization, function. Pantoea agglomerans is a dominant gut bacterium that colonizes the gut of. Highquality draft genome sequence of a new phytaseproducing.